What is the first step in carrying out chromatography?

The first step in carrying out chromatography is to place spots of ink or dye on a pencil line. This step is essential because it establishes the initial position of the substances to be separated. The samples are typically applied as small spots or lines on a stationary phase, such as chromatography paper, before any solvent is introduced. The placement of the samples allows them to be adequately analyzed as the solvent moves up the paper, carrying the components of the mixture with it. Proper application of the samples is crucial for achieving effective separation during the chromatography process. Once the spots are applied, the next steps, such as adding the solvent, can proceed.